【文献解读】调节Wnt /β-连环蛋白信号通路对毛囊干细胞的定向分化的影响

【文献解读】调节Wnt /β-连环蛋白信号通路对毛囊干细胞的定向分化的影响
2017/9/30 创新医学网

     文章来源：欧尔意 OAE 微信号

     作者：Sofina

     原文出处

     Yang B, Wu XY, Ni J, Li BH, Deng LH, Xiang MJ. Modulation of Wnt/β-catenin signaling affects the directional differentiation of hair follicle stem cells. Plast Aesthet Res 2016;3:39-46.

     DOI: 10.20517/2347-9264.2015.75

     摘要

     目的：毛囊干细胞(HFSCs)可分化成毛囊细胞，在皮肤烧伤的治疗中具有的潜在临床应用价值。然而，调节HFSCs分化为毛囊乳头或表皮细胞的机制目前还不清楚。本研究调查了Wnt /β-连环蛋白通路及其与其他信号通路交互作用在这一分化过程中的作用。方法：采用氯化锂(LiCl，10 mmol / L)和角质形成细胞生长因子(KGF，10μg/ L)诱导HFSC分化，通过免疫荧光分析验证。测量3,5,7天和9天后β-连环蛋白，结肠腺瘤样息肉蛋白，糖原合成酶激酶-3β(GSK-3β)，axin和淋巴增强因子-1的mRNA表达，以评估Wnt的作用/β-连环蛋白信号通路的作用。结果：在LiCl诱导的HFSC分化为毛囊细胞期间，Wnt /β-连环蛋白信号通路被激活，并且GSK-3β(降解化合物的重要成分)的表达被抑制。这导致细胞质β-连环蛋白表达增加，核易位和随后的靶基因转录。相比之下，KGF诱导HFSC分化为表皮细胞，并不影响β-连环蛋白的表达。该数据表明，LiCl和KGF均具有调节HFSCs分化为毛囊和表皮细胞的作用。此外，Wnt /β-连环蛋白信号通路主要参与毛囊分化。结论：这些结果表明，LiCl可用于在体外将HFSC分化为毛囊细胞，其在治疗皮肤损伤中具有的重要的治疗应用。

     关键词：氯化锂，角质形成细胞生长因子，毛囊干细胞，Wnt /β-连环蛋白信号通路

     Aim:The differentiation of hair follicle stem cells (HFSCs) into hair follicle cells has potential clinical applications for cutaneous burns. However, the mechanisms regulating the differentiation of HFSCs into hair follicular papilla or epidermal cells are currently not clear. This study investigated the role of the Wnt/β-catenin pathway and its crosstalk with other signaling components during this differentiation process. Methods:Lithium chloride (LiCl, 10 mmol/L) and keratinocyte growth factor (KGF, 10 μg/L) were used to induce HFSC differentiation, validated by immunofluorescence analysis. The mRNA expression of β-catenin, adenomatous polyposis coli, glycogen synthase kinase-3β (GSK-3β), axin, and lymphoid enhancer factor-1 after 3, 5, 7, and 9 days were measured to evaluate the role of the Wnt/β-catenin pathway. Results:During LiCl-induced HFSC differentiation into hair follicle cells, the Wnt/β-catenin signaling pathway was activated and the expression of GSK-3β, a vital component of the degradation compound, was inhibited. This led to increased cytoplasmic β-catenin expression, nuclear translocation, and subsequent target gene transcription. By contrast, KGF induced the differentiation of HFSCs into epidermal cells and did not affect the expression of β-catenin. This data indicates that LiCl and KGF distinctly regulate the differentiation of HFSCs into hair follicle and epidermal cells, respectively. Furthermore, the Wnt/β-catenin signaling pathway is predominantly involved in hair follicle differentiation.

     Conclusion: these results demonstrate that LiCl can be used to differentiate HFSCs into hair follicle cells in vitro, which has important therapeutic applications for treating patients with cutaneous damage.

     Keywords:Lithium chloride; keratinocyte growth factor; hair follicle stem cells; Wnt/β-catenin signaling pathway

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     本文摘要由浙江大学医学院第二附属医院烧伤科中心 任海涛教授翻译提供，中文内容仅供参考，一切以英文原版为主。

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